Research Assistant in Peter Gorer Department of Immunobiology at King’s College London

Salary: Grade 5, £34,502 – £36,348 per annum, including London Weighting Allowance

Building and Campus: Guy’s Hospital, Bermondsey/Southwark Wing, Guy’s Campus

Contact: Annika Warnatsch, Annika.warnatsch@kcl.ac.uk

Hiring manager/s: Denise Jamieson, Rianna Nicholas

Job description

Are you looking to gain extensive research experience before undertaking a PhD or a career in Industry? Why not consider a Research Assistant role?

This is an exciting opportunity for a Research Assistant to study the role of neutrophil-derived reactive oxygen production in antiviral inflammation. Specifically, the project will analyze the regulation of innate and adaptive immune responses through Reactive Oxygen Species (ROS) localization during respiratory virus infections such as influenza A virus and SARS-CoV-2.

We have recently acquired novel data showing that neutrophils account for the majority of ROS production during the early phase of Influenza A virus infection which in turn modulates protective T cell responses as well as release of cytokines and inflammatory mediators.

The purpose of the study is to investigate the timing and localization of neutrophil ROS production during respiratory virus infection using airway epithelial cultures as well as the effects of neutrophil ROS production on the activation of innate and adaptive immune responses and the resolution of inflammation.

You will have an honors degree (minimum 2:1) or an MSc in a relevant biological science. Some experience in virology combined with cell culture, flow cytometry, biochemical or virological assays is desirable. Previous experience with confocal microscopy is an advantage. You will have an interest in tackling fundamental questions regarding viral pathogenesis and inflammation, will work independently with support from the PI and the research group.

The position is available immediately in the laboratory of Dr. Annika Warnatsch, Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, Faculty of Life Sciences and Medicine on Guy’s campus. For an informal discussion or to find out more about the role please contact: annika.warnatsch@kcl.ac.uk.

Contract type

This post will be offered on a fixed-term contract for 20 months

This is a full-time post – 100% full time equivalent

Key responsibilities

  • Perform in vitro and/or in vivo infection experiments with lab-attenuated influenza A virus (Containment level 2) and virus quantification
  • Implement protein biochemistry, gene expression analysis, flow cytometry and immunofluorescence approaches to study effects of neutrophil ROS signaling on antiviral inflammation
  • Live imaging using confocal microscopy to understand timing and localization of neutrophil ROS signaling upon virus infection
  • Processing of human blood and isolation of PBMCs and neutrophils
  • Maintenance of transgenic mouse strains
  • Organization and record keeping
  • Maintenance of resources and reagents

The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.

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