ALS Gene Therapy Technician at King’s College London

Location: Denmark Hill Campus

Contact details: Younbok Lee, younbok.lee@kcl.ac.uk

Job description

The successful candidate will be part of a team testing viral vector gene therapy reagents aimed at treating amyotrophic lateral sclerosis (ALS) and fronto-temporal dementia (FTD) on a commercially funded project. The aim of the project is to optimize the dose and delivery of gene supplement and knock-down vectors in cellular and animal models of disease as proof of concept and efficacy for potential clinical application. Successful candidates will join a team of technicians and post-doctoral scientists analyzing cells and tissues transduced with plasmid and vector constructs and quantify the effects on target gene expression. For in vivo work the candidate will sacrifice animals and harvest and prepare tissues for histological sectioning, staining, imaging and quantitative analyses. All experiments will be undertaken at a very high standard and all data treated with complete confidentiality.

This post will be offered on a fixed-term contract until 30th September 2024
This is a full-time post – 100% full time equivalent

Key responsibilities

  • Perform laboratory work to optimize the efficiency of our preclinical studies.
  • Perform high level of molecular biology (Cloning), RT-PCR, Western blots and maintaining cell culture.
  • Deliver high-quality data from our preclinical studies including:

– Undertake rodent colony care, breeding and genotyping

– primary neuron culture

  • Optimize perfusion, harvesting, fixation and sectioning of animal tissues.
  • Image tissues using slide scanning and confocal microscopes
  • Undertake image analysis using advanced analytical software tools

The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.

Skills, knowledge, and experience

  • In vivo and In vitro experimental methods
  • Rodent colony maintenance and genotyping
  • Tissue collection and processing for immunohistochemistry and biochemistry
  • Advanced microscopy using confocal imaging
  • Quantitative image analytical software and statistical analyses
  • AAV gene transduction and expression

Essential criteria

  1. BSc in Neuroscience or equivalent biological field
  2. An ability to work independently and within a team
  3. Attention to detail and high standards of accuracy in all work
  4. Conscientious and organized
  5. Ability to manage time appropriately
  6. Willing to work occasionally during weekends and bank holidays

Desirable criteria

  1. Experience of AAV gene therapy

Further information

For queries, please contact Dr Younbok Lee

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